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Huh7 lm3

Web1 May 2016 · Finally, we constructed the reporter gene with ATF3 promoter linked to luciferase reporter and assayed its transcriptional activity after altering the TR4 expression in Huh7 cells and LM3 cells (Figure 6H, 6I). The results proved the transcription activation of ATF3 was induced by TR4 (Figure 6E–6I). WebHuh7 and MHCCLM3 cells which were transfected with si-NC and si-SNHG4, respectively, were harvested with RIPA lysis buffer containing 1 mM Na 3 VO 4, 1 mM Cocktail and 1 mM PMSF. Cell debris was removed by centrifugation. The total protein concentration was determined by the BCA protein assay.

TR4 nuclear receptor enhances the cisplatin chemo-sensitivity via ...

Web青蒿琥酯与索拉非尼组合在制备AKTc-Myc激活的肝癌Huh7细胞药物的应用 [P]. 中国专利: CN110025618A . 2024-07-19 WebThe Huh7, LM3 and Hep3B cell lines have high invasive characteristics, whereas the YY‑8103, SNU‑398 and MHCC‑97L cell lines have less invasive characteristics (18). … dhl servicepoint sassenheim https://loken-engineering.com

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WebIn different HCC cell lines (Hep3B, Huh7, PLC8024, and HepG2), ALDH activity shows at 89%, 49%, 46%, and 8% of cells, respectively. The isolated ALDH + HCC cells present chemoresistance. Dual-color flow cytometry analysis showed the majority of ALDH + HCC cells to be CD133 +. WebThree types of HCC cells (LM3, Hep G2, Huh7) were treated with sorafenib, doxorubicin hydro-chloride, and cisplatin. We found that the lipid profiles of HCC cells changed a lot … http://yuxiqbs.cqvip.com/Qikan/Search/Index?key=K%3d%e5%be%ae%e5%b0%8fRNA-26a cilium without kube-proxy

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Huh7 lm3

Direct targeting sperm-associated antigen 9 by miR-141 …

Web9 Apr 2024 · And CD79B and TNFRSF11B were significantly down-regulated in MHCC97H, Huh7 and LM3, while S100A9 was up-regulated. Conclusion We constructed an IRGs … WebLow-metastatic HepB3 and LM3 cells were treated with Huh7-derived exosomes, miR-4800-3p mimic, or miR-4800-3p inhibitor for 48 h, respectively. The proliferation and stemness …

Huh7 lm3

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Web专业的医学、医疗、药学、生命科学知识搜索引擎。涵盖医学内容搜索、丁香园论坛搜索、丁香人才网职位搜索、试剂耗材搜索、丁香博客搜索,最新资讯等内容 Web23 Oct 2024 · Complete methylation was found in SNU182, SNU449, HBXF344, SMMC7721, Huh7, HepG2, LM3, PLC/PRF/5, and BEL7402 cells, partial methylation …

WebHepatocellular carcinoma cell lines (Hep3B, SMMC-7721, LM3 and Huh7) and L02 were purchased from the American Type Culture Collection (ATCC, Manassas, VA), and cultured in complete culture medium (HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA), 100 U/ml penicillin (Gibco), and 100 … Web4 Mar 2024 · The TCF4 expression plasmid pcDNA3.1-TCF4 was generated by insertion of the full-length ORF of TCF4 into the Kpn I and Xho I sites of pcDNA3.1 vector …

Web18 Sep 2024 · For transfection, Huh7 or LM3 cells were plated into 6-well plates and incubated for 24 h. Then, the cells were transfected with pcDNA3.1-KCNQ1 using … Web23 Oct 2024 · HCC cell lines, which included SNU182, SNU387, HBXF344, SNU475, HepG2, PLC/PRF/5, Huh7, BEL7402, LM3, SNU449, SMMC7721, QGY7703, and Huh1 …

Web1 May 2016 · Finally, we constructed the reporter gene with ATF3 promoter linked to luciferase reporter and assayed its transcriptional activity after altering the TR4 …

WebHepatocellular carcinoma (HCC) is the world's third leading cause of cancer death, with metastasis being the predominant cause of treatment failure. We aim to explore the role of Talin1 in HCC and its underlying mechanism. We conducted bioinformatic analysis, qRT-PCR, Immunohi... cilium with 9 2 axonemeWeb22 Mar 2024 · The Sox15 overexpression vector or lentivirus was transfected into the Huh7, SK-hep-1, and LM3 cells using polybrene (8 µg/mL, Sigma). The overexpression … dhl servicepoint teteringenWeb18 Sep 2024 · (A) Efficiency of RAB34 siRNA in LM3 and Huh7 cell lines. (B) Results of the CCK-8 assay in LM3 and Huh7 cell lines transfected with the RAB34 siRNA or negative … ciliving.tv contestWebh22细胞稳转接瘤. 各位老师好!最近在用dkk1慢病毒稳转h22细胞,先抽了腹水,每个皿种了200万细胞进行稳转,48小时后荧光挺多,就接到小鼠体内继续长,想着能够扩增多一点的细胞用于接瘤,一周后剖开鼠,发现对照组腹水. 丁香园-药理及临床试验-2024-07-04 15:49:42 dhl servicepoint tilburg noordWeb15 Sep 2024 · Human HCC cell lines, including LM3, Huh7, PLC/PRF/5, and Hep3B were purchased from the Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences, Shanghai, China). HCC cell lines were incubated in DMEM (Gibco, Gaithersburg) with 10% fetal bovine serum and maintained at 37℃ in a humidified incubator with … cilix berlinWeb9 May 2014 · In the present study, we used salinomycin (Sal) and 5-fluorouracil (5-FU) combination therapy on HCC cell lines Huh7, LM3 and SMMC-7721 and nude mice … dhl servicepoint winschotenWebAmong them, The HMMR upregulation were the most significant in LM3 and Huh7 cell lines. Similar results were obtained by using Western blotting analysis (Figure 3B). Figure 3: … cilix disease