Hepg2 puromycin
Web嘌呤霉素 (Puromycin)是一种由白黑链霉菌产生的氨基苷类抗生素，是氨酰-tRNA分子3´末端的类似物，能够与核糖体的A位点结合并掺入到延伸的肽链中，打乱核糖体上的肽转运，造成翻译过程中不成熟链终止，从而抑制蛋白质合成。 图1嘌呤霉素结构式 (CAS NO. 53-79-2) 嘌呤霉素产生菌Streptomyces alboniger内发现的pac基因编码嘌呤霉素N-乙酰转移酶 … http://hydranal.cnreagent.com/product_74496.html
Hepg2 puromycin
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Web11 apr. 2024 · VIP36-KO HepG2 cells were similarly transfected, and 24 h after transfection, the complexes were replaced with medium containing 2 μg/mL puromycin (#anti-pr-1, InvivoGen, Toulouse, France) to establish a stably transfected cell line. WebPuromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram-positive …
Web25 sep. 2024 · The HepG2 cells and HepG2-puro cells served as the naïve cell control and the transfection system control, respectively. (B) HBcAg synthesis in HBV-infected HepG2-SBP cells was detected by IFAs. HBcAg was stained with an anti-HBcAg mAb (red), and the nucleus was stained with DAPI (blue). Web11 apr. 2024 · Cell lines and cell culture. Human HCC cell lines MHCC-97H, HepG2, and HEK293T were obtained from the American Type Culture Collection. These cells were tested for mycoplasma contamination and cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (TIANHANG) and 1% …
WebAfter 24 hours, the stably infected SNU-387 cell was selected with 2 μg/mL of Puromycin 2HCL (Selleck). The TMPO-AS1 expression vector (pcDNA3.1-TMPO-AS1, named as pc-TMPO-AS1) and empty pcDNA3.1 vector (negative control, named as pc-vector) ... Next, Huh7 and HepG2 cells were selected to construct TMPO-AS1-overexpressing cell lines. WebTRAIP promotes malignant behaviors and correlates with poor prognosis in liver cancer. Biomed Pharmacother. 2024 Apr;124:109857. doi: 10.1016/j.biopha.2024.109857. Epub 2024 Jan 20.
Websince Puromycin inhibits protein synthesis, it's always better to keep the cells in low confluence so that they are in the growing phase. Normally Puromycin acts quite well …
http://www.biofeng.com/xibao/xibaozhu/Hepa1-6-eGFP-Puro.html blake snow real estateWebSpandidos Publications blakes nm 528 lotaburger rio rancho nmWeb7 uur geleden · Successfully transduced cells were selected with 10 µg ml −1 (293T) or 50 µg ml−1 (HepG2) puromycin (AdipoGen Life Science). ArgRS knockdown was confirmed via western blot and quantitative ... blakes north perthWeb22 feb. 2024 · Mitochondrial depolarization was similar in wild type, VDAC1/2, 1/3, and 2/3 double KO HepG2 cells indicating that the effect of SC18 was not VDAC isoform-dependent. framemaker insert special charactersWebLuciferase Hep G2细胞稳定表达 绿色荧光蛋白标记 。. 该细胞株性状稳定，培养时不需要添加抗生素维持。. 可用作 绿色荧光蛋白标记 活性检测中的阳性对照，也可用于活体动物成像实验。. Hep G2细胞是来自15岁男性白人的组织；Hep G2细胞形态为上皮细胞样，模式 ... blakes nukeproof scoutWebPuromycin (Sigma-Aldrich Co., St Louis, MO, USA) at 2 μg/mL was added to each well to select transfection cells. Ethics statement and tissues samples. We recruited 50 cases of HCC tissues in this study, and 20 cases of adjacent nontumor liver tissues were used as control. ... Notes: (A) Total proteins from HepG2 cells were extracted, ... blakes north main las crucesWebPuromycin工作浓度筛选预实验 大部分细胞puromycin的工作浓度为1~10μg/ml在《慢病毒感染手册》上查阅Puromycin目的细胞中筛选的致死筛选参考用量。 部分细胞的参考用 … blakes north weald